Coding
BclA-His

Part:BBa_K1933000:Design

Designed by: Tomoki Uchino   Group: iGEM16_Kyoto   (2016-10-10)


N-terminal domain of BclA protein fused to 6xHis tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codons were optimized for expression in E. coli K-12 strain.

Source

NCBI; Accession number CAD56880, CAD56878, CAD56869, and CAD56870
DNA was chemically synthesized.

References

[1]Park, Tae Jung, et al. "Surface display of recombinant proteins on Escherichia coli by BclA exosporium of Bacillus anthracis. " Microbial cell factories 12.1 (2013): 1.